Radioimmunoassay for Gram-negative bacterial lipopolysaccharide O antigens: influence of antigen solubility
نویسندگان
چکیده
منابع مشابه
Radioimmunoassay for Gram-negative bacterial lipopolysaccharide O antigens: influence of antigen solubility.
We have developed a solid-phase radioimmunoassay technique for specific gram-negative bacterial lipopolysaccharide (LPS) O antigens. The method exploits the high-titer, specific immunoglobulin M response of the rabbit to LPS immunization to measure as little as 5 ng of homologous LPS per ml with less than 0.5% cross-reactivity toward heterologous LPS or culture supernatants. We found that O ant...
متن کاملTransport of lipopolysaccharide to the Gram-negative bacterial cell surface.
Lipopolysaccharides (LPS) are major lipidic components of the outer membrane of most Gram-negative bacteria. They form a permeability barrier that protects these bacteria from harmful compounds in the environment. In addition, they are important signaling molecules for the innate immune system. The mechanism of transport of these molecules to the bacterial cell surface has remained enigmatic fo...
متن کاملC1 inhibitor prevents Gram-negative bacterial lipopolysaccharide-induced vascular permeability.
Gram-negative bacterial endotoxemia may lead to the pathological increase of vascular permeability with systemic vascular collapse, a vascular leak syndrome, multiple organ failure (MOF), and/or shock. Previous studies demonstrated that C1 inhibitor (C1INH) protects mice from lipopolysaccharide (LPS)-induced lethal septic shock via a direct interaction with LPS. Here, we report that C1INH block...
متن کاملMechanisms of O-Antigen Structural Variation of Bacterial Lipopolysaccharide (LPS)
One of the most complex topics within bacterial anatomy and physiology is that of exopolysaccharides. These molecules have various structures and functions and also provide different types of advantages to their producing microorganisms, including surface variability, resistance to innate and acquired immunity mechanisms, the ability to adhere to different surface and cell types and resistance ...
متن کاملO-Antigen Protects Gram-Negative Bacteria from Histone Killing
Beyond their traditional role of wrapping DNA, histones display antibacterial activity to Gram-negative and -positive bacteria. To identify bacterial components that allow survival to a histone challenge, we selected resistant bacteria from homologous Escherichia coli libraries that harbor plasmids carrying pieces of the chromosome in different sizes. We identified genes required for exopolysac...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1979
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.26.1.42-48.1979